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KMID : 0881720160310040278
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Journal of Food Hygiene and Safety
2016 Volume.31 No. 4 p.278 ~ p.285
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Optimization of Extraction Conditions for Mixture of Camellia sinensis L. and Artemisia argyi by Response Surface Methodology
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Kim Young-Hyun
Kim Jae-Min
Choi Sun-Il
Jung Tae-Dong
Lee Jin-Ha
Kim Jong-Dai
Lee Ok-Hwan
Kim Woo-Sik
Lim Jae-Kag
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Abstract
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This study used response surface methodology (RSM) in an effort to optimize the ultrasoundassisted extraction condition of Camellia sinensis L. and Artemisia argyi mixture in order to increase extraction yield in the extract. The effects of three independent variables, X1 (Mixture ratio, 60-80%), X2 (Ratio of water to raw material, 20~100 mL/g), and X3 (Extraction time, 25-145 min), were investigated at three levels using Box-Behnken design (BBD) to obtain the highest extraction efficiency. Y (Extraction yield) was chosen as dependent variable. Our result showed that the coefficient of determination (R2) of the model was 0.9747, with significant at the level of p < 0.002. Furthermore, the predicted values of each variable were similar to the actual values. The optimum extraction conditions were as follows: mixture ratio of 85.86%, ratio of water to raw material of 92.73 mL/g, and extraction time of 56.52 min. At these conditions, predicted extraction yield was 30.03%. The analysis of variance (ANOVA) indicated a high goodness of model fit and the success of the RSM method for optimizing extraction conditions of Camellia sinensis L. and Artemisia argyi mixture.
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KEYWORD
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Response surface methodology, Ultrasound-assisted extracted, Box-Behnken design, Camellia sinensis L., Artemisia argyi
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